TACE Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
TACE
Enzyme
TACE, human recombinant (residues 1-671) expressed in insect cell with His tag
Synonym
A disintegrin and metalloproteinase domain 17, TNF-alpha-converting enzyme, TNF-alpha convertase, Snake venom-like protease, CD156b antigen
Substrates
(5-FAM/QXLTM) FRET peptide
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (M) |
|---|---|
| NNGH | 6.88E-07 |
| Actinonin | 1.14E-06 |
| GM6001 | 3.03E-08 |
| MMP Inhibitor III | 2.22E-08 |
| MMP-9/MMP-13 Inhibitor I | 1.99E-09 |
Scientific Information
P78536 (http://www.uniprot.org/uniprot/P78536)
Screening Location
Malvern, USA
Reference compound IC50 for TACE
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