Activity-based TAK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TAK1
Enzyme
Human TAK1
HGNC
MAP3K7
Synonym
TGF1a, MEKK7
Substrates
Casein, 1 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 36 |
| Ro 31-8220 | 16,700 |
| GW 5074 | 14,000 |
Scientific Information
O43318 (http://www.uniprot.org/uniprot/O43318)
Screening Location
Malvern, USA
Reference compound IC50 for TAK1
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