Activity-based TBK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TBK1
Enzyme
Human TBK1
HGNC
TBK1
Synonym
CDC37, HSPCA, HSPCB, IKBKG, NAK, T2K, TANK, TRAF2
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.3 |
| Ro 31-8220 | 340 |
| GW 5074 | 11,200 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
Q9UHD2 (http://www.uniprot.org/uniprot/Q9UHD2)
Screening Location
Malvern, USA
Reference compound IC50 for TBK1
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