Activity-based TEC kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TEC
Enzyme
Human TEC
HGNC
TEC
Synonym
PSCTK4
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 52.7 |
| PP2 | 425 |
| AG1478 | 25,000 |
| PDGFR I-III | >30,000 |
| D-64406 | 15,000 |
Scientific Information
P42680 (http://www.uniprot.org/uniprot/P42680)
Screening Location
Malvern, USA
Reference compound IC50 for TEC
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