Activity-based TESK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TESK1
Enzyme
Human TESK1
HGNC
TESK1
Synonym
TESK1
Substrates
Cofilin 2, 3 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 260 |
| Ro 31-8220 | 22,000 |
| GW 5074 | 7,500 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
Q15569 (http://www.uniprot.org/uniprot/Q15569)
Screening Location
Malvern, USA
Reference compound IC50 for TESK1
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