Activity-based TGFBR1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
TGFBR1
Enzyme
Human TGFBR1, wt
HGNC
TGFBR1
Manning ID
TGFbR1
Synonym
TGFB-R1, ALK5, ACVRLK4, TGFBR1, ACVRLK4, ALK5/TGFBR1
UniProt
P36897 (TGFR1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TKL
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-GSK3(14-27), ATP
Reaction
RBER-GSK3(14-27) + 33P-ATP = 33P-RBER-GSK3(14-27) + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 453 |
| Sorafenib | 10000 |
| Sunitinib | 51000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | TGFBR1 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – TGFBR1 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0397-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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