Thrombin Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
Thrombin
Enzyme
Alpha Thrombin
Species
Human plasma
Synonyms
Factor IIa
Scientific Information
P00747 (https://www.uniprot.org/uniprotkb/P00747)
Assay Properties
Substrates
Fluorogenic peptide H-D-CHA-Ala-Arg-AMC 2AcOH, 10 µM
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Gabexate Mesylate | 1470 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Protease Assays
Reference compound inhibition of Thrombin
PDF