TIE2 (Y1108F) Kinase Assay Service

Activity-based TIE2 (Y1108F) kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

TIE2 (Y1108F)

Enzyme

Human TIE2 (Y1108F)

HGNC

TEK (Y1108F)

Synonym

VMCM, TIE-2, VMCM1, CD202B

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q02763 (http://www.uniprot.org/uniprot/Q02763)

Screening Location

Malvern, USA

Reference compound IC50 for TIE2 (Y1108F)