TLK2 Kinase Assay Service - Reaction Biology

Activity-based TLK2 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

TLK2

Enzyme

Human TLK2

HGNC

TLK2

Synonym

MGC44450, PKU-ALPHA

Substrates

Casein, 1 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
Ro 31-8220	2,500
Staurosporine	5.7

Scientific Information

Q86UE8 (http://www.uniprot.org/uniprot/Q86UE8)

Screening Location

Malvern, USA

Reference compound IC50 for TLK2