TRIM33b-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-TRIM33b-H
Protein
Human TRIM33b bromodomain, His-tagged
Synonyms
E3 ubiquitin-protein ligase TRIM33; Ectodermin homolog; Protein Rfg7; TIF1-gamma; Tripartite motif-containing protein 33
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.075 mg/ml (3.1 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
50,5
Reference Compound ΔTms:
Bromosporine – 1.0 (Blue)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, SGC-CBP30, BET151, GSK2801 and PFI3
Scientific Information
Q9UPN9 (http://www.uniprot.org/uniprot/Q9UPN9)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
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