Activity-based TRKA (G667A) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TRKA (G667A)
Enzyme
Human TRKA (G667A)
HGNC
NTRK1 (G667A)
Synonym
High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor A, NTRK1
Scientific Information
P04629 (http://www.uniprot.org/uniprot/P04629)
Assay Properties
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.1 |
| PP2 | 780 |
| AG1478 | 1,200 |
| EGFR/Erb Inhibitor | >100,000 |
| D-64406 | 85 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for TRKA (G667A)
