Activity-based TRKA G667C kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
TRKA G667C
Enzyme
Human TRKA G667C, mutant
HGNC
NTRK1 (G667C)
Manning ID
TRKA
Synonym
MTC, TRK1, TRKA, TRK-A G667C, TRK, TRKA G667C, TRKA (G667C)
UniProt
P04629 (NTRK1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 3 |
| Sorafenib | 6 |
| Sunitinib | 1300 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | TRKA G667C |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – TRKA G667C (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1537-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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