Activity-based TRKB kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
TRKB
Enzyme
Human TRKB, wt
HGNC
NTRK2
Manning ID
TRKB
Synonym
TRK-B, TRKB, Trk-B, NTRK2, GP145-TrkB
UniProt
Q16620 (NTRK2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 4 |
| Sorafenib | 1500 |
| Sunitinib | 110 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | TRKB |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – TRKB (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0686-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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