Activity-based TSSK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TSSK4
Enzyme
Human TSSK4
HGNC
TSSK4
Synonym
Testis-specific serine/threonine-protein kinase 4, Serine/threonine-protein kinase 22E, C14orf20, STK22E, TSSK5
Scientific Information
Q6SA08 (http://www.uniprot.org/uniprot/Q6SA08)
Assay Properties
Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 13 |
| Ro 31-8220 | 70 |
| GW5074 | 11,000 |
| H-89 | 1,300 |
| Rottlerin | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for TSSK4
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