Activity-based TYK2 JH1 & JH2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TYK2 JH1 & JH2
Enzyme
Human TYK2 JH1 & JH2
HGNC
TYK2
Synonym
Non-receptor tyrosine-protein kinase TYK2
Scientific Information
P29597 (http://www.uniprot.org/uniprot/P29597)
Assay Properties
Substrates
Peptide substrate, [KKSRGDYMTMQIG], 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.55 |
| PP2 | 13,000 |
| AG1478 | 53,000 |
| EGFR/ErbB Inhibitor | >100,000 |
| D-64406 | 2,700 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for TYK2 JH1 & JH2
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