TYRO3/SKY Kinase Assay Service

Activity-based TYRO3/SKY kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

TYRO3/SKY

Enzyme

Human TYRO3/SKY

HGNC

TYRO3

Synonym

BYK, Brt, Dtk, Sky, Tif

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q06418 (http://www.uniprot.org/uniprot/Q06418)

Screening Location

Malvern, USA

Reference compound IC50 for TYRO3/SKY