UCHL3 Ubiquitin-Proteasome Pathway Assay Service

UCHL3 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview

CAT#

UCHL3

Enzyme

Human UCHL3

Synonym

Ubiquitin carboxyl-terminal hydrolase isozyme L3, Ubiquitin thioesterase L3, UCH-L3

Substrate

Fluorogenic substrate [Ubiquitin-AMC], 100 nM

Assay Properties

Reaction

Cleavage of ubiquitin for release of fluorescent AMC

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (µM)
UCHL1 I 6.26
UIPase I >200
UI1 157

Scientific Information

P15374 (http://www.uniprot.org/uniprot/P15374)

Screening Location

Malvern, USA

Reference compound IC50 for UCHL3

Reference compound IC50 for UCHL3