Activity-based ULK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ULK1
Enzyme
Human ULK1
HGNC
ULK1
Synonym
UNC51, Unc51.1
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
O75385 (http://www.uniprot.org/uniprot/O75385)
Screening Location
Malvern, USA
Reference compound IC50 for ULK1
