USP14 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP14
Enzyme
Human USP14
Synonym
Ubiquitin carboxyl-terminal hydrolase 14, Deubiquitinating enzyme 14, Ubiquitin thiolesterase 14, Ubiquitin-specific-processing protease 14, TGT, UBP14
Substrate
Fluorogenic substrate [Ubiquitin-AMC], 100 nM
Assay Properties
Reaction
Cleavage of ubiquitin for release of fluorescent AMC
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCHL1 I | 72.4 |
| Ub Aldehyde | 2.8 |
| UCHL1 2 | 76.5 |
Scientific Information
P54578 (http://www.uniprot.org/uniprot/P54578)
Screening Location
Malvern, USA
Reference Compound IC50 for USP14
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