USP15 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP15
Enzyme
Human USP15
Synonym
Ubiquitin carboxyl-terminal hydrolase 15, KIAA0529, Unph-2, Unph4
Scientific Information
Q9Y4E8 (https://www.uniprot.org/uniprotkb/Q9Y4E8)
Assay Properties
Substrate
Ubiquitin Rhodamine 110 Protein, 100 nM
Reaction
Ubiquitin Rhodamine 110 = Ubiquitin + Rhodamine 110
Rhodamine 110 fluorescence, kinetic measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCH-L3 Inhibitor | 10.9 |
| UCH-L1 Inhibitor 2 | 52.0 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Deubiquitination Assays (DUB assays)
Reference Compound Inhibition for USP15
PDF