USP25 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP25
Enzyme
Human USP25
Synonym
Ubiquitin carboxyl-terminal hydrolase 25, USP21
Scientific Information
Q9UHP3 (https://www.uniprot.org/uniprotkb/Q9UHP3)
Assay Properties
Substrate
100 nM Ubiquitin Rhodamine 110 Protein
Reaction
Ubiquitin Rhodamine 110 = Ubiquitin + Rhodamine 110
Rhodamine 110 fluorescence, kinetic measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| AZ1 | 0.33 |
| UCH-L1 Inhibitor | 6.4 |
| UCH-L1 Inhibitor 2 | 28.3 |
| UCH-L3 Inhibitor | 15.8 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Deubiquitination Assays (DUB assays)
Reference Compound IC50 for USP25
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