USP4 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP4
Enzyme
Human USP4
Synonym
UNP, UNPH, Ubiquitin carboxyl-terminal hydrolase 4, Deubiquitinating enzyme 4, Ubiquitin thioesterase 4, Ubiquitin-specific-processing protease 4, Ubiquitous nuclear protein homolog
Substrate
Ubiquitin Rhodamine 110 Protein, 100 nM
Assay Properties
Reaction
Ubiquitin Rhodamine 110 = Ubiquitin + Rhodamine 110
Rhodamine 110 fluorescence, kinetic measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| Vialinin A | 4.01 |
| UCH-L3 Inhibitor | 8.2 |
| UCH-L1 Inhibitor | 55.4 |
| UCH-L1 Inhibitor II | 52.9 |
Scientific Information
Q13107 (https://www.uniprot.org/uniprotkb/Q13107/entry)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Deubiquitination Assays (DUB assays)
Reference Compound IC50 for USP4
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