USP5 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP5
Enzyme
Human USP5
Synonym
Ubiquitin carboxyl-terminal hydrolase 5, Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thiolesterase 5, Ubiquitin-specific-processing protease 5, ISOT, UBP5
Substrate
Fluorogenic substrate [Ubiquitin-AMC], 100 nM
Assay Properties
Reaction
Cleavage of ubiquitin for release of fluorescent AMC
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCHL1 I | 34.4 |
| UIPase I | 126 |
| UI1 | >32.8 |
Scientific Information
P45974 (http://www.uniprot.org/uniprot/P45974)
Screening Location
Malvern, USA
Reference Compound IC50 for USP5
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