Activity-based VEGFR1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
VEGFR1
Enzyme
Human VEGFR1, wt
HGNC
FLT1
Manning ID
FLT1
Synonym
VEGF-R1, FLT, VEGFR1, FLT1, FLT1/VEGFR1
UniProt
P17948 (VGFR1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 43 |
| Sorafenib | 1900 |
| Sunitinib | 580 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | VEGFR1 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – VEGFR1 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0097-0000-3 |
| Web Shop | Reaction Biology Online Shop |
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