Activity-based VEGFR3 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
VEGFR3
Enzyme
Human VEGFR3, wt
HGNC
FLT4
Manning ID
FLT4
Synonym
VEGF-R3, PCL, VEGFR3, FLT4, FLT4/VEGFR3
UniProt
P35916 (VGFR3_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 70 |
| Sorafenib | 640 |
| Sunitinb | 340 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | VEGFR3 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – VEGFR3 |
| Product Number | 0225-0000-2 |
| Web Shop | Reaction Biology Online Shop |
PDF