Activity-based VRK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
VRK2
Enzyme
Human VRK2
HGNC
VRK2
Synonym
VRK2
Substrates
Casein, 2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
Q86Y07 (http://www.uniprot.org/uniprot/Q86Y07)
Screening Location
Malvern, USA
Reference compound IC50 for VRK2