Activity-based WEE2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
WEE2
Enzyme
Human WEE2
HGNC
WEE2
Synonym
Wee1-like protein kinase 2, Wee1B kinase, WEE1B
Scientific Information
P0C1S8 (http://www.uniprot.org/uniprot/P0C1S8)
Assay Properties
Substrates
Protein substrate, CDK1 (D146N)/cyclin B1, 5 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2,400 |
| PP2 | >100,000 |
| AG1478 | 16,000 |
| WEE1 Inhibitor | 16 |
| D-64406 | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for WEE2
