Activity-based WNK1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
WNK1
Enzyme
Human WNK1, wt
HGNC
WNK1
Manning ID
Wnk1
Synonym
KDP, KIAA0344, PHA2C, PRKWNK1, WNK1
UniProt
Q9H4A3 (WNK1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
Other
Assay Properties
Assay Format
33PanQinaseTM
Substrates
3xChocktide-PKC(19-31), ATP
Reaction
3xChocktide-PKC(19-31) + 33P-ATP = 33P-3xChocktide-PKC(19-31) + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| WNK463 | 72 |
| Sorafenib | 10000 |
| Sunitinib | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | WNK1 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – WNK1 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1111-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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