WNK1 Kinase Assay Service - Reaction Biology

Activity-based WNK1 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

WNK1

Enzyme

Human WNK1

HGNC

WNK1

Synonym

KDP, KIAA0344, PHA2C, PRKWNK1

Substrates

MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q9H4A3 (http://www.uniprot.org/uniprot/Q9H4A3)

Screening Location

Malvern, USA

Reference compound IC50 for WNK1