Activity-based WNK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
WNK2
Enzyme
Human WNK2
HGNC
WNK2
Synonym
KIAA1760, NY-CO-43, P/OKcl.13, PRKWNK2, SDCCAG43
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
Q9Y3S1 (http://www.uniprot.org/uniprot/Q9Y3S1)
Screening Location
Malvern, USA
Reference compound IC50 for WNK2
