YES1 (T348I) Kinase Assay Service

Activity-based YES1 (T348I) kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

YES1 (T348I)

Enzyme

Human YES1 (T348I)

HGNC

YES1 (T348I)

Synonym

C-YES, HsT441, P61-YES, YES1

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

P07947 (http://www.uniprot.org/uniprot/P07947)

Screening Location

Malvern, USA

Reference compound IC50 for YES1 (T348I)