YTHDF2 Reader Domain Assay for compound screening and profiling using the HTRF assay format to measure compound binding to the target.
Target Overview
CAT#
YTHDF2
Enzyme
Human YTHDF2 (401-554)
Synonyms
HGRG8, CLL-associated antigen KW-14, High-glucose-regulated protein 8, Renal carcinoma antigen NY-REN-2
Scientific Information
Q9Y5A9 (www.uniprot.org/uniprotkb/Q9Y5A9)
Assay Properties
Ligand
Biotinylated ssDNA probe with m6-A modification
5’-/5Biosg/GTTGCCTGTTCGTGTTGG/iN6Me-dA/CTTGCCTGT-3’
Reaction
Binding interaction between YTHDF2 and Biotinylated ssDNA probe with m6-A modification
Readout
HTRF
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| ssDNA probe with m6-A modification (5’-GTTGCCTGTTCGTGTTGG/iN6Me-dA/CTTGCCTGT-3’) |
95.7 |
Screening Location
Malvern, PA, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Reference competition inhibition of YTHDF2 and Biotinylated ssDNA probe with m6-A modification binding interaction
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