Activity-based ZAK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
ZAK
Enzyme
Human ZAK, wt
HGNC
MAP3K20
Manning ID
ZAK
Synonym
MLTK, mlklak, AZK, ZAK, MAP3K20, ZAK/MLTK
UniProt
Q9NYL2 (M3K20_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TKL
Assay Properties
Assay Format
33PanQinaseTM
Substrates
MBP, ATP
Reaction
MBP + 33P-ATP = 33P-MBP + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Sorafenib | 240 |
| Sunitinib | 76000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | ZAK |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | No |
| Product Number | N/A |
| Web Shop | Reaction Biology Online Shop |
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