Activity-based ZIPK/DAPK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ZIPK
Enzyme
Human ZIPK
HGNC
DAPK3
Synonym
ZIPK/DAPK3, ZIP, ZIPK
Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
O43293 (http://www.uniprot.org/uniprot/O43293)
Screening Location
Malvern, USA
Reference compound IC50 for ZIPK/DAPK3