ProLiFiler – Cell Panel Screening Service

The ProLiFiler service is suitable for testing of the therapeutic efficacy of test compounds on a panel of 140 human cancer cell lines using CellTiter-Glo readout.

Cell line panel screening provides efficacy data for 140 tumor cell lines from across 18 tumor types to identify the most responsive tumor entities and determine which cell lines are best suited for subsequent studies with more sophisticated 3D cell culture models or animal studies, all of which can be performed with Reaction Biology.

  • Performed on a regular basis
  • Huge cost-savings
  • Fast turnaround time: get your results in only 6 weeks
  • Option for in-depth bioinformatics analysis

Service Details

Assay Description

assay principle of the cellular proliferation assay performed with celltiter-glo

Cells were seeded in cell culture-treated 384 multi-well plates in the respective medium (A). After overnight incubation at 37 °C, compounds are dispensed by a Nanodrop Tecan D300e machine (B). Cells are treated for 72 h at 37°C at 5 % or 10% CO2 (C). Finally, cell plates are equilibrated to room temperature and the viability of cells is determined with CellTiter-Glo luminescent reagent based on the amount of ATP present (D).
 

Reproducibility

reproducibility cell proliferation and viability assay on large panel screen

The semi-automated screening process produces highly reproducible data. In every run a reference compound serves as the control reference compound. Shown here are the mean data and standard error of results from 4 independent ProLiFiler runs.

Case Study - efficacy in tumor cell lines

met kinase inhibitor on large cell line panel screen

Identification of cell lines sensitive to a MET kinase inhibitor. The MET inhibitor was tested for anti-proliferative and cytotoxic capacity with the ProLiFiler cell line panel. Three cell lines were most receptive to treatment including MKN-45, LOVO, and Karpas 299.

Case Study - Tumor Origins

tumor entity water fall blot after large cell line panel screen

Identification of the tumor origin most sensitive to a MET kinase inhibitor. IC50 values of the inhibitor potency on a panel of tumor cell lines were sorted based on the tumor entity. Colon tumors (red) were most susceptible to treatment.

Bioinformatic Analysis

Our bioinformatics analysis tools are available to identify the mechanism of action of your test compound and to detect suitable biomarkers for stratification of responders vs. non-responders.

MOA Finder

Biomarker Analysis

To offer these analytic tools, we have partnered with 4HF Biotec GmbH, a bioinformatics firm specializing in cancer data mining to discover new anti-cancer drugs. 4HF Biotec performs the data mining analysis based on their proprietary database and provides hands-on support with a high focus on client communication to answer your key questions.

Assay Setup and Schedule

Setup: IC50 determination or single concentration testing with CellTiter-Glo readout. Drug combination testing is also an option, see an example study here.

Controls: DMSO only treatment serves as high control. Staurosporine treatment (at 1x10-5M) serves as the low control. In every project, the IC50 value determination of a reference compound is included.

Report: A detailed report including assay conditions, methodology, and comprehensive evaluation of data as well as raw data for each analysis will be provided.

Screening schedule: Please find the screening schedule here. Results are available after 6 weeks.

Testing of subsets: For testing a subset of cell lines, or single concentration screening of multiple compounds, please see our Cell Proliferation Assay service with the free choice setup here.

Screening Facility: The ProLiFiler cell line panel screening is performed in Freiburg, Germany.

Compound requirements: In brief: 100 µl of the stock solution with 1000x the highest testing concentration or the equivalent as solid material.