Isocitrate Dehydrogenase Assay Services for Drug Discovery

Isocitrate dehydrogenase assays are suited for compound screening and profiling. IDH1 and IDH2 are key metabolic enzymes that catalyze the conversion of isocitrate to a-ketoglutarate (aKG) and cofactor NADPH. Our IDH assays are based on measuring enzyme activity in a coupled system wherein NADPH produced in the initial reaction is a co-factor in the conversion of resazurin to fluorescent resorufin in a secondary reaction.

Low and large scale as well as high-throughput IDH screening options are available
Readout: Isocitrate dehydrogenase enzymatic activity
Custom-tailored IDH assay development possible
All IDH enzymes are produced in house and available for purchase

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List of IDH Assays (11)

Target	Synonym	Data
IDH1 G97D	IDH1 G97D	Data sheet
IDH1 R100A	IDH1 R100A	Data sheet
IDH1 R100Q	IDH1 R100Q	Data sheet
IDH1 R132C	IDH1 R132C	Data sheet
IDH1 R132H	IDH1 R132H	Data sheet
IDH1 WT	IDH1	Data sheet
IDH1 Y139D	IDH1 Y139D	Data sheet
IDH2 R140K	IDH2 R140K	Data sheet
IDH2 R140Q	IDH2 R140Q	Data sheet
IDH2 R172Q	IDH2 R172Q	Data sheet
IDH2 WT	IDH2	Data sheet

Assay Details

Assay Format

Diaphorase/resazurin-coupled assay

Depending on the mutation status IDH either produces or consumes NADPH. Quantifying the amount of NADPH is therefore a readout for enzyme activity.
For readout, diaphorase utilizes NADPH to convert the dye resazurin to resorufin, a molecule fluorescent at 580 nm.

Assay setup

Setups: Single-dose screening in duplicates or IC50 value determination with 5 or 10 concentrations. Other screening formats are available upon request.

Controls: No inhibitor (DMSO vehicle) control and for every assay, one target-specific control compound is tested in 10-dose IC50 format.

Turnaround time: 10 business days for standard projects. Expedited scheduling and data delivery can be arranged prior to the commencement of the studies.

Report: The raw data, % enzyme activity and control compound IC50 values will be reported in Excel format for single-dose assays. For IC50 orders, raw data, IC50 values, and curve fitting will be delivered in Excel format. Assay conditions, target, and substrate information are available upon request. Requirements for this information should be noted prior to the commencement of the study.

Screening facility: This assay is performed at our screening facility in Malvern, PA, US.

Compound requirements: In brief, for a standard project, 20 µl of a 10 mM DMSO stock or solid material is needed. Less material is needed for large scale screening. Please refer to our FAQs for information regarding compound preparation and shipping.

Please see our FAQ page to access our assay condition form (US facility).

Isocitrate Dehydrogenase Assay Services for Drug Discovery

List of IDH Assays (11)

Assay Details

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