Protein Production and Assay Development

Reaction Biology can assist you with the production of proteins and assays for a variety of target classes such as kinases, epigenetics, PARPs, DUB, HSP, protease, phosphatase, and many more.

Our services comprise:

  • Small or large scale custom-tailored protein production
  • Assay development and optimization/validation
  • Enzyme testing, substrate testing, and activator/inhibitor testing
Protein Production
Expression systems
  • Sf9 and Sf21 insect cells
  • E.coli
Available tags
  • GST
  • HIS
  • GST-HIS
  • HALO-HIS
  • SUMO-HIS
  • TXN-HIS
  • Additional tags are available
Modification
  • Activation by autophosphorylation
  • Activation by an upstream kinase
  • Removal of tag
  • Co-expression with co-factors
  • Mutations

 

Assay Development

Radiometric detection including:

  • 3H
  • 33P
  • 32P


Fluorescence-based detection methods such as:

  • AlphaLISA
  • AlphaScreen
  • Fluorescence intensity
  • Fluorescence polarization
  • FRET
  • TR-FRET


Luminescence-based assays such as:

  • Bioluminescence, such as ADP-Glo and ATP bioluminescence-based assays
  • Chemiluminescence, such as horseradish peroxidase (HRP) and alkaline phosphatase (AP) based assays


Absorbance and colorimetric based assays


For biophysical detections we offer:

 

For cell-based detections we offer:

  • NanoBRET technology
  • Calcium mobilization
  • Second messenger detection
  • Enzymatic activity via substrate detection by using ELISA or Western blotting or Glo-based assays
  • TR-FRET-based competitive immunoassay
  • Electrophysiologic readouts via patch clamp technique
  • Gene expression quantification via luciferase reporter assays