KinaseFinder Screening Service

The KinaseFinder is a biochemical assay intended to identify kinases, which accept a given substrate for phosphorylation.

The KinaseFinder is a standardized service package for identifying protein kinases that can phosphorylate a peptide or protein of interest provided by our customer. Kinase hits, identified with the standard package, should subsequently be confirmed in a second experiment. The KinaseFinder is ideal for the elucidation of physiological pathways with the involvement of protein kinases.

Potential substrates can be peptides or proteins
Compare peptides with phospho-site mutations
Option to follow up with an SDS-PAGE and autoradiogram to visualize the phosphorylated substrate

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List of Kinase Panels

List of Kinase Panels
Type of KinaseFinder	# of kinases	Assay format
Custom panel	selected by customer	HotSpot™
Ser/Thr & Tyr protein kinases	339	³³PanQinase™
Ser/Thr protein kinases	245	³³PanQinase™
Tyr protein kinases	94	³³PanQinase™
Wild Type Kinase Panel	380	HotSpot™

Assay Details

Example Data

Example data with 50 Ser/Thr kinases.

Activity of kinases with example substrate protein are shown (corrected for substrate background activity). Substrate protein was tested at one concentration. Yellow: Kinase with substrate protein; blue: Kinase without substrate protein (autophosphorylation). A ratio value between substrate phosphorylation and apparent autophosphorylation > 3 may be considered as significant. Performed with the ³³PanQinase™ assay. cpm = counts per minute

Screening Details

Setups: Proteins and peptides are accepted as substrates.

Assay format: radiometric screening with ³³P-ATP as phosphate donor

Turnaround time: 10 business days for standard projects. Expedited scheduling and data delivery can be arranged prior to commencement of the studies.

Screening location: Assays run with the ³³PanQinase™ assay are performed at the German site. Assays run with the HotSpot™ assay are performed at the US site.

Please see our FAQ page to access our biochemical assay condition form (German facility) for further details on substrate preparation and shipping.

Reference Publication

Corvin Walter et al. employed the Kinase Finder service to identify kinases that phosphorylate the TOM70, which represents the main import receptor for the carrier import pathway. Kinases DYRK1A and DYRK1B scored highest in phosphorylating TOM70 in the primary Kinase Finder screen (Fig 1a). The hits were confirmed by using increasing concentrations of substrate (Supp Fig. 1b). To confirm the DYRK1 specific phosphorylation site, a TOM70 mutant was used for screening which resulted in significantly less phosphorylation (Supp. Fig. 1g).

Walter et al., Global kinome profiling reveals DYRK1A as critical activator of the human mitochondrial import machinery

KinaseFinder Screening Service

KinaseFinder Assay Principle

List of Kinase Panels

Assay Details

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