Deubiquitination Assays (DUB assays)
The ubiquitin-proteasome pathway is responsible for the degradation of intracellular proteins as part of the general protein turnover, regulation of gene transcription, and protein abundance in cells as well as quality control. Proteins are being ubiquitylated to mark them for degradation by proteasomes. This process is highly regulated by ubiquitin-ligases and deubiquitinases. Ubiquitin is expressed as a fusion protein precursor that needs to be processed by deubiquitinating enzymes to yield monomers.
20S Proteasome Activity Assay measures the chymotrypsin-like activity by cleaving the fluorescent substrate Suc-LLVY-AMC.
- Low-scale, large-scale and high-throughput DUB screening options are available
- Please ask us for deubiquitinase assay development and custom-tailored options
- Deubiquitinase enzymatic activity is measured with Ubiquitin-AMC fluorescent substrate
