Nuclear Receptor Assay Services

Nuclear receptors are transcription factors that are regulated by small hydrophobic ligands such as hormones or vitamins. Upon ligand binding, nuclear receptors translocate into the nucleus to bind DNA and modulate the expression of their target genes to regulate a variety of cellular mechanisms such as growth, proliferation, metabolism, or homeostasis on a transcriptional level. Nuclear receptors are successfully targeted in the clinic to treat, for example, cancer, cardiovascular or autoimmune diseases.

Reaction Biology offers a suite of assays to support the discovery of new drugs to modulate nuclear receptors with the following advantages:

• Cell-based assays allow for drug testing in the physiological and complex environment of intact cells
• Our luciferase reporter assay format measures the activity of the nuclear receptors on the target gene expression, therefore, a large variety of drugs can be tested such as modulators of translocation, activity, structural changes, dimerization, etc.
• We offer low and large scale drug screening