Histone Acetyltransferase Assay (HAT) Services for Drug Discovery

Histone acetyltransferase assays performed at Reaction Biology are radiometric activity assays using tritiated acetyl-Coenzyme A as a cofactor.

Reaction Biology’s HAT assays are radioisotope-based filtration assays. This gold standard approach can be used regardless of whether the substrate is a peptide, histone, nucleosome, or non-histone protein. A radiolabeled acetyl group is transferred to the substrate to quantify enzyme activity.

With ~1750 lysine-acetylated proteins in the human proteome, Reaction Biology’s histone acetyltransferase assays provide an important advantage in terms of assay flexibility as well as gold standard quality.

Direct and accurate measurement of histone acetyltransferase activity
Low scale, large scale, and high-throughput HAT inhibitor screening options
Histone acetyltransferase assay development and research collaboration options

All our histone acetyltransferase assay services are performed at our screening facility in Pennsylvania, US. Our team of Ph.D. scientists provide quality data and excellent communication, for a superior research result.

Business development managers in your area answer to your HAT assay screening inquiries to support your specific research needs. Please expect standard reports to be delivered within 10 business days. Coordinate with your business development manager for expedited services.

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HAT Inhibitor Screening Details

Target	Synonyms	UniProt	Data
CBP	CREBBP	Q92793	Data sheet
KAT2A	hGCN5	Q92830	Data sheet
KAT2B	pCAF	Q92831	Data sheet
KAT5	TIP60	Q92993	Data sheet
KAT6A	MYST3	Q92794	Data sheet
KAT6B	MYST4	Q8WYB5	Data sheet
KAT7	MYST2	O95251	Data sheet
KAT8	MYST1	Q9H7Z6	Data sheet
p300	EP300	Q09472	Data sheet

Assay format

Histone acetyltransferases acetylate lysines on histones and other proteins using tritium-labeled acetyl-Coenzyme A as the acetyl donor. The tritium-acetyl group is transferred onto the histone substrate that is measured directly to reflect the enzyme activity.

Assay setup

Setups: Single-dose screening in duplicates or IC50 value determination with 5 or 10 concentrations. Other screening formats are available upon request.

Controls: No inhibitor (DMSO vehicle) control and for every assay, one target-specific control compound is tested in 10-dose IC50 format.

Turnaround time: 10 business days for standard projects. Expedited scheduling and data delivery can be arranged prior to the commencement of the studies.

Report: The raw data, % enzyme activity and control compound IC50 values will be reported in Excel format for single-dose assays. For IC50 orders, raw data, IC50 values, and curve fitting will be delivered in Excel format. Assay conditions, target, and substrate information are available upon request. Requirements for this information should be noted prior to the commencement of the study.

Screening facility: This assay is performed at our screening facility in Malvern, PA, US.

Compound requirements: In brief, for a standard project, 20 µl of a 10 mM DMSO stock or solid material is needed. Less material is needed for large scale screening. Please refer to our FAQs for information regarding compound preparation and shipping.

Please see our FAQ page to access our assay condition form (US facility).