MoA Finder Tool
In collaboration with the bioinformatics firm 4HF Biotech GmbH which is a bioinformatics firm specializing in cancer data mining to discover new anti-cancer drugs, we offer data mining for identification or confirmation of the mechanism of action of a customer compound.
The MoA Finder tool aims to identify the mode of action (MoA) of your test compound based on the results obtained in the ProLiFiler cell panel screening. To this end, we compare the sensitivity profile of your drug candidate to more than 700 drugs for which the MoA is known.
- The MoA Finder helps to identify potential off-targets and side effects.
- Potential new indications can be found for an established drug.
- The identification of compounds with similar MoA gives suggestions for target deconvolution in a phenotypic approach.
Additional Information
Primary data set: We will perform the ProLiFiler assay for testing the anti-proliferative effects of your drug on a set of 140 human tumor cell lines for IC50 determination. This is called the sensitivity profile of your compound.
Database: Our collaborator 4HF Biotech has built a comprehensive database, including proprietary and literature data of the sensitivity profile of more than 700 anti-cancer compounds integrated into a single platform for visualization and statistical analysis.
Analysis: Scientists of the 4HF Biotech team will compare the sensitivity profile of your drug to that of hundreds of reference drugs with known mechanism of action. The correlation of the two sensitivity profiles is evaluated by the Spearman Rho correlation value and its p-value.
Deliverables: The report comprises a graphical presentation of the correlation analysis and all raw data. 4HF Biotech scientists are available for a phone conversation to walk you through the analysis, data, and results focussing on getting your key questions answered.
Example of doxorubicin in the MoA Finder analysis. Shown is a graphical presentation of the correlation of the drug sensitivity profile of doxorubicin to 732 reference drug sensitivity profiles. Each dot represents one correlation. The dots' size is related to the number of cell lines used to define the reference drug's sensitivity profile. A positive correlation of doxorubicin and the drug is blue; a negative correlation is shown in red. Those reference drugs with a Spearman Rho correlation higher than 0.7 are named.
The top 10 hits for the MoA Finder performed with doxorubicin are presented in a table. Of the 9 hits next to doxorubicin are 8 inhibitors of DNA replication and cell-cycle regulation. Teniposide and etoposide are both topoisomerase II inhibitors confirming the MoA of our tool compound doxorubicin which is also a topoisomerase II inhibitor.
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